Changes in cell surface molecules associated with in vitro culture of prostatic stromal cells

The unique characteristics of cell surface molecules on human bone marrow stromal cells (hBMSCs) have not been clearly elucidated. The authors characterized 19 cell surface molecules on culture-expanded hBMSCs obtained from 10 human donors, by flow cytometry, calculated the averages and standard deviations of the expression frequencies of individual surface molecules, and evaluated their expression patterns with respect to donor-dependent variations. Surface molecules expressed at frequencies of more than 80% on cells included, CD49e, CD29, CD90, CD73, CD44, CD105, and CD146, those expressed at frequencies of 20-80% included, Stro-1, CD51, CD54, CD31, CD106, CD163, and CD34, whereas CD49d, CD14, CD144, CD11b, and CD45 were expressed at less than 20%. Substantial donor-dependent variations (>10% standard deviation in expression percentage) were observed in the expressions of CD105, Stro-1, CD51, CD54, CD31, CD106, CD163, and CD34. Our results demonstrate that CD49e, CD29, CD90, CD73, and CD44 can be used as a positive marker for hBMSCs, and that CD144, CD11b, and CD45 could be used as a highly selective negative marker. However, the expressions of CD54, CD31, and CD106 observed in the present study, contradicted prior reports and thus, their expressions remain controversial.

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Cytoadherence-related homologous motifs inPlasmodium falciparumantigen Pf155/RESA and erythrocyte band 3 protein

Parasitology ◽

10.1017/s0031182000065215 ◽

1995 ◽

Vol 110 (5) ◽

pp. 503-511 ◽

Cited By ~ 9

Author(s):

J. Iqbal ◽

A. B. Siddique ◽

N. Ahlborg ◽

P. Perlmann ◽

K. Berzins

Keyword(s):

Plasmodium Falciparum ◽

Cell Surface ◽

Synthetic Peptides ◽

Molecular Basis ◽

Band 3 ◽

Cell Surface Molecules ◽

Band 3 Protein ◽

Surface Molecules ◽

Erythrocyte Antigen

SUMMARYCytoadherence ofPlasmodium falciparum-infected erythrocytes plays an important role in the pathogenesis of cerebral malaria. The identity of cell surface molecules on parasitized erythrocytes involved in cytoadherence is of great interest to understand the molecular basis of this mechanism. Peptide sequences derived from exofacial loops of the erythrocyte antigen band 3 from parasitized erythrocytes have previously been shown to inhibit cytoadherence. We now report that a non-repeated region of Pf155/RESA (residues 213–218) contains a hexapeptide motif being highly homologous to cytoadherence inhibitory sequences from band 3. Synthetic peptides containing the hexapeptide motif of Pf155/RESA inhibited the binding ofP.falciparum-infected erythrocytes to melanoma cellsin vitro. Furthermore, individuals residing in malaria-endemic areas have antibodies reactive with epitopes involving these motifs in band 3 and in Pf155/RESA.

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Cell surface molecules of peripheral blood mononuclear cells adhered to cultured human keratinocytes in vitro

Journal of Dermatological Science ◽

10.1016/0923-1811(94)90394-8 ◽

1994 ◽

Vol 8 (1) ◽

pp. 66

Author(s):

I. Matsuyama ◽

K. Urano ◽

R. Urano ◽

I. Matsuo

Keyword(s):

Cell Surface ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Keratinocytes ◽

Cell Surface Molecules ◽

Peripheral Blood Mononuclear ◽

Surface Molecules ◽

Blood Mononuclear Cells

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Cell surface molecules in human CD34+ progenitor-derived dendritic cells as markers for in vitro sensitization

Toxicology Letters ◽

10.1016/j.toxlet.2007.05.246 ◽

2007 ◽

Vol 172 ◽

pp. S88

Author(s):

Inge Nelissen ◽

Geert R. Verheyen ◽

Nathalie Lambrechts ◽

Daniella Ooms ◽

Hilde Leppens ◽

...

Keyword(s):

Dendritic Cells ◽

Cell Surface ◽

Cell Surface Molecules ◽

Surface Molecules ◽

Human Cd34

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Role of leukemia inhibitor factor (LIF) in decidualisation of murine uterine stromal cells in vitro

Journal of Endocrinology ◽

10.1677/joe.0.1810477 ◽

2004 ◽

Vol 181 (3) ◽

pp. 477-492 ◽

Cited By ~ 19

Author(s):

AA Fouladi Nashta ◽

CV Andreu ◽

N Nijjar ◽

JK Heath ◽

SJ Kimber

Keyword(s):

In Vitro Culture ◽

Stromal Cells ◽

Control Level ◽

Calf Serum ◽

Prostaglandin E ◽

Dependent Manner ◽

Alp Activity ◽

Dose Dependent ◽

In Cells

Decidualisation of uterine stromal cells is a prerequisite for implantation of the embryo in mice. Here we have used an in vitro culture system in which stromal cells decidualise as indicated by a number of markers, including an increase in alkaline phosphatase (ALP) activity. The latter was used as a quantitative marker of decidualisation in the presence of low (2%) fetal calf serum. Prostaglandin E(2) (PGE(2)), which is known to induce decidualisation, increased ALP activity, and this effect was blocked in a dose-dependent manner by indomethacin. Leukemia inhibitory factor (LIF) was then examined, but it had no effect on PGE(2) secretion. However, LIF suppressed ALP activity in a dose-dependent manner in the presence of 2% serum, while an inhibitor of LIF that competes for binding to its receptor reversed the effect of LIF and increased ALP activity above the control level. In serum-free cultures, stromal cells differentiated rapidly, and no differences were observed between LIF-treated and untreated cultures. Stromal cells produce LIF during in vitro culture, and this peaked at 48 h. Freshly collected stromal cells from both day-2 and -4 pregnant mice expressed mRNA for the LIF receptor, and the transcript level was higher in cells isolated on day 4. However, no differences were observed in the relative levels of transcripts in cells from day 2 and day 4 after culture, nor were there differences between the LIF-treated cultures and controls. Therefore, in this study, we have shown that LIF suppresses decidualisation of murine uterine stromal cells in the presence of serum, this is not due to the regulation of PGE(2) secretion by stromal cells.

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